TOPlistReconstitution, calculated dose and application Peptides -
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1.Definition Reconstitution

The peptides are supplied as a lyophilized powder. The amount of powder (the product) is referred to in International Units (IU) or milligrams (mg). The reconstitution of the lyophilized powder the process of dilution with a solvent. As the solvent, sodium chloride 0.9% (NaCl), 0.6% acetic acid (AA) or bacteriostatic water (BA).

Example no.1

SOLUTIONS FOR PEPTIDES: Sterile Bacteriostatic Water (BA) or a solution of 0.9% sodium chloride (NaCl)

• It is recommended to be used to reconstitute the peptide Melanotan 2, PT-141 (Bremelanotide), BPC-157, Thymosin Beta 4 (TB-500), CJC-1295 DAC +, ModGRF 1-29 (CJC-1295 without DAC) Sermorelin (GRF 1-29), GHRP-2, GHRP-6, ipamorelin, Hexarelin HGH fragment 176-191, idyll, oxytocin, and Epithalon DSIP.

SOLUTIONS FOR PROTEINS: A solution of 0.6% acetic acid (AA)

• It is recommended to be used for reconstitution, such as proteins. (IGF-1 LR3, DES 1-3 Mecasermin).

2.Methodology Reconstitution

STEP 1: Attach an alcohol wipe the vial closure (plastic or glass bottle containing lyophilisate) and peptide vial with solvent (solution).

Step 2: Load the recommended solvent into the syringe with the desired volume (eg. 1-2 ml). Choose an amount of solvent to facilitate the calculation of the dose of the finished product upon dilution.

STEP 3: Take the solvent syringe, push it into the vial of lyophilized powder and allow the solvent to dissolve the peptide. Be careful, because many (but not all) peptides are vacuum sealed.

STEP 4: After the addition of the solvent into the vial gently rotate the vial until the lyophilized powder is dissolved. The peptide is now ready for use. In the event that the peptide immediately after dilution was completely dissolved (solution containing undissolved particles floating / fragments), first make sure that you have added to the vial enough of a solution.

STEP 5: If you want the new reconstituted peptide administered immediately after reconstitution, store in a refrigerator at 2-8 ° C. The vast majority of the peptides in solution may be stable at this temperature for 2-3 weeks. If the reconstituted peptide is not going to be taken immediately after dilution, store in a freezer at -20 ° C. At this temperature, the peptides may be storage stable in solution for 3 months.

Example no.2

The individual segments (units) at the syringe is virtually point, respectively. signs that we see the scale of the syringe.

• 2 ml (2 cc) syringe on his scale of 200 pieces.
• 1 ml (1 cc) syringe on his scale 100 divisions.
• 0.5 ml (0.5 cc) syringe on his scale 50 pieces.
• 0.3 ml (0.3 cc) syringe on his scale 30 pieces.

3.Calculation Benefits for Application

After a successful reconstitution of the peptide calculate the amount recommended dose for subcutaneous (under the skin) injection. Using a syringe, draw the desired amount of dose vials containing reconstituted peptide and then apply your product. For successful calculated amount of benefits will be necessary to know the following relationship: 1 ml = 100 pieces.

Example no.3

Since we know the amount of the peptide (mg / mcg or IU) in a vial, and we can divide it as follows:

• We used 1 ml of a 10 mg vial peptide.
• We know that 1 ml = 100 pieces.
• Earn 100 divisions (solution) 10 mg (peptide).
• Result: 100 pieces / 10 mg = 10 pieces.
• Every 10 pieces on the syringe will be equal to 1 mg peptide.

Need to stretch a dose of 2 mg peptide?

• Withdraw the reconstituted peptide comma after 20 (division / unit) on a scale syringe.
• The value is 1/5 syringes containing 1 ml.

Example no.4

• If you have a 1 mg vial and add 1 ml of solution, you get
1000 mcg / 100 pieces = 10 mcg to 1 division syringe
• If you have a 1 mg vial and add 2 ml solution, you get
1000 mcg / 200 pieces = 5 mcg to 1 division syringe
• If you have 10 mg vial and add 1 ml of solution, you get
10 mg / 100 divisions = 1 to 10 mg pieces of the syringe
• If you have 10 mg vial and add 2 ml solution, you get
10 mg / 200 mg = 0.5 divisions to 10 divisions of the syringe
• If you have 20 mg vial and add 1 ml of solution, you get
20 mg / 100 pieces = 2 mg per 10 pieces syringe
• If you have 20 mg vial and add 2 ml solution, you get
20 mg / 200 mg, 1 division = 10 pieces in the syringe

Example no.5

• If you have a 10 IU vial and add 1 ml of solution, you get
10 IU / 1 100 IU pieces = 10 pieces in the syringe (product 1/10)
• If you have a 10 IU vial and add 2 ml solution, you get
10 IU / 1 200 IU pieces = 20 pieces in the syringe (also product 1/10)
• If you have a 5000 IU vial and add 1 ml of solution, you get
5000 IU / 100 pieces = 500 IU per 10 pieces syringe
• If you have a 5000 IU vial and add 2 ml solution, you get
5000 IU / 200 pieces = 250 IU per 10 pieces syringe

4. Procedure for calculating the levy

STEP 1: Determine the total amount of product in vials in mg / mcg / IU.

STEP 2: Select the amount of solvent (solution) that you add to a vial of lyophilized powder peptide.

STEP 3: divide the total amount of product in vials in mg / mcg / IU value of the amount of solvent in pieces.

STEP 4: The result is the amount of peptide, which will include one scale interval on your syringe after stretching.

STEP 5: The required amount of the recommended dose of the peptide divide the quantities that may contain 1 increment (number given in step 4). The resulting value is the number of divisions of the syringe, you need to stretch to the desired volume of the recommended dose of the peptide.

5. Definition of Subcutaneous injectables

Subcutaneous injection is the incorporation of the substance into the subcutaneous tissue to achieve the desired effect, depending on the administered dose. To achieve the desired effect of the peptides is sufficient subcutaneous administration (the fat / points between the top layer of skin and muscle) using the insulin. Intramuscular injection (into a muscle) is not necessary. The most commonly injected subcutaneous injection into the abdomen (belly fat). Report carried Subcutaneous insulin injection into the abdominal fat is painless and simple. Application site is easily accessible, visible and achievable.

6. Method of Application

STEP 1: Always wash your hands thoroughly.

STEP 2: Prepare the syringe.

• Remove the needle from the protective cap, top and bottom.
• Pull the plunger of the syringe to draw the amount of air equivalent to the dose you want to stretch.
• Insert the needle into the vial through the center of the rubber stopper.
• Inject the air into the vial. Leave the needle in the vial.
• Turn the vial on the contrary, the needle must be kept in vials.
• Again, pull the syringe plunger up to the desired mark on the needle.
• Check the needle for air bubbles. Tap the needle to the bubbles move to the surface and push the plunger to remove them.

STEP 3: Select the injection site.

• You can apply anywhere on the body where fat is higher.
• Always vary the injection site.
• Never apply to bruised, swollen or scratching posts.

STEP 4: Clean the injection site.

• an alcohol swab, wipe the area two inches of the selected injection site.
• Allow the area to dry.

STEP 5: Injecting.

• hand that you write, hold the needle over the disinfected.
• With the other hand press the area two or three inches on one side of the cleaned site.
• A quick motion, push the needle into the pinched point. The needle must go all the skin.
• Push the plunger until all the contents of the syringe.
• Pull the needle.
• alcohol swab gently wipe the injection site.

7. Unit Conversions

ML (ml = milliliter). It is a unit of volume, which is 1/1000 liters. When referring to water or other fluids, this unit is equivalent to one cubic centimeter.

Cc (cc = cubic centimeter). It's another volume. In most cases the capacity is measured in cc syringes. If you have a syringe of 1 cc, keep 1 ml of liquid.

IU (IU = international units). This unit is used to measure the activity (ie effect) of many vitamins and drugs. For each substance to which this unit, there is an international agreement specifying the biological effect expected dose of 1 IU. Other quantities shall be expressed as multiples of this standard. This means that the ECU is based only on the volume or mass of material, but rather its effect.

MG (mg = milligrams). It is a unit of weight, which is one thousandth of a gram. Quantities of a chemical is often measured in milligrams. The solutions for administration by injection is referred to as the concentration ratio of weight to volume, such as mg / ml (milligrams per milliliter). In the case of orally administered substances, the weight of the substance indicated, although the actual weight of the tablet / capsule can be much higher due to the use of filler substances. Thus, the small pill may be much more effective than the bolus. Therefore neposudzujte pills based on their size, but the actual amount of that substance.

MCG (mcg / mg = microgram = 1/1000 mg). One milligram contains thousands of micrograms.

Example no.6


1000 mcg / mg (micrograms) = 1 mg (milligram)
1000 mg (milligrams) = 1 g (gram)
1000 g (grams) 1 kg (kilogram)
1000 ml (milliliter) = 1 L (liter)
1 ml (milliliter) = 1 cc (cubic centimeter)


How to use subcutaneous injection -

How to use intramuscular injection -